Efficient nuclear FISH on paraffin-embedded tissue sections using microwave pretreatment.
نویسندگان
چکیده
منابع مشابه
Single-day FISH procedure for paraffin-embedded tissue sections using a microwave oven.
The fluorescence in situ hybridization (FISH) technique is a sensitive method for detecting gene amplification in tumor cells. However, FISH on formalin-fixed and paraffin-embedded tissue sections is time-consuming and labor-intensive (1). Tissue processing and cross-linking fixatives such as formalin may prevent the probe from accessing its target sequence, prolonging the FISH processing time ...
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عنوان ژورنال:
- BioTechniques
دوره 26 3 شماره
صفحات -
تاریخ انتشار 1999